Intracellular recording of the membrane potential of a pyramidal neuron in the CA1 region of the hippocampus (bottom), and extracellular recording of the CA1 pyramidal layer local field potential (top) during a sharp-wave ripple event. Recorded from a freely behaving mouse. In orange is a different CA1 pyramidal neuron filled with biocytin during intracellular recording, and in blue DAPI staining
I am a neurophysiologist currently training as a postdoctoral fellow in the laboratory of Dr. Gyorgy Buzsaki at New York University. Previously I received my PhD from Rutgers University under the mentorship of Dr. Tibor Koos and Dr. James M. Tepper.
I am interested in neural circuits, and how synaptic, cellular and network physiology interact to produce precisely timed neuronal activity in support of behavior.
For my PhD thesis I combined optogenetic techniques with in vitro whole cell recordings and in vivo single unit recordings to discover novel cholinergic-GABAergic microcircuitry in the neostriatum.
GABAergic circuits mediate the reinforcement-related signals of striatal cholinergic interneurons
English et al. 2012 Nature Neuroscience
My first postdoctoral project investigated sharp-wave ripple oscillations in the hippocampus in vivo, from the perspective of the membrane potential of single pyramidal neurons. I applied intracellular sharp electrode recording techniques to freely moving mice, and used this novel approach to demonstrate how excitatory and inhibitory inputs to CA1 pyramidal neurons compete during sharp-wave ripple oscillations to control spiking.
Excitation and inhibition compete to control spiking during hippocampal ripples: intracellular study in behaving mice
English et al. 2014 Journal of Neuroscience
My second postdoctoral project investigated the architecture and dynamics of pyramidal cell-interneuron circuits in vivo in awake/sleeping mice. I developed a hybrid juxtacellular-silicon probe design which enabled me to record and stimulate single pyramidal neurons while recording ~40 local circuit neurons, including interneurons, in awake/sleeping head fixed mice. I utilized this preparation, along with ultra-focal optogenetic stimulation and silicon probe recording of pyramidal cells and interneurons in freely behaving mice, to demonstrate that short-latency peaks in spike train correlations can be used to identify monosynaptic pyramidal cell-interneuron connections. I utilized this method of synapse detection to examine the anatomy and physiology of the CA1 pyramidal cell-interneuron circuit, with a special attention to features relevant to construction of transiently active cell assemblies.
Under review (Neuron) - Click here to see the preprint on Cell Press Sneak Peek
My Google Scholar
Link to Buzsaki Lab Website: http://www.buzsakilab.com